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KMID : 0613820040140060967
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Journal of Life Science
2004 Volume.14 No. 6 p.967 ~ p.974
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Effects of Rubus coreanus Miquel Extracts on the Activity and Differentiation of MC3T3-E1 Osteoblastic cell
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Lee Ji-Won
Lee In-Seon
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Abstract
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The osteoblastic cell activity is important for born formation, thus, this study was performed to investigation of that the effect of edible sources, Rubus coreanus Miquel (RCM), on the proliferation and differentiation of MC3T3-E1 osteoblastic like cell. The effects of RCM extract on cell proliferation were measured by MTT assay. At 1, 10 μg/mL of RCM extract treated, that were elevated of cell proliferation to 103 and 142% via control, respectively. And the cell differentiation were measured as alkaline phosphatase (ALP) activity at 3, 9, 18, and 27 days. As the results, the 10 μg/mL was increased ALP activity more than 2.6 times compared with control, 1.4 times via positive control at 27th day (p<0.05). The optical concentration of RC extract was rechecked by ALP staining and Alizarin Red staining for investigation of the induction of ALP activity, nodule formation by mineralization. mRNA expression analysis showed that the RCM (10μg/mL) increased in SOX9 as well as ALP in MC3T3-E1 cells. These results suggest that RC extract was stimulates the MC3T3-E1 cell proliferation and differentiation.
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KEYWORD
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Rubus coreanus Miquel, osteoblast, bone formation, proliferation, alkaline phosphatase, mineralization
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